HELICOBACTER PYLORI ANTIBODY TEST - detection from blood

HELICOBACTER PYLORI ANTIBODY DETECTION - from the serum mainly

Helicobacter Pylori is a gram-negative bacterium that causes inflammation of the stomach lining. The results of affection by this bacterium may lead to the chronic gastritis and is strongly linked to the development of duodenal and gastric ulcers and stomach cancer. 

Helicobacter pylori antibody detection from serum/plasma/whole blood is a rapid qualitative test usually based on the immunochromatographic principle. In this test procedure, anti-human IgG is immobilized in the test line region; after specimen addition, it reacts with the H. pylori antigen coated particles in the test. This mixture migrates chromatographically along the length of the test and interacts with immobilized anti-human IgG. 

If the specimen contains H. pylori antibodies, a coloured line will appear in the test line region indicating a positive result. If the specimen does not contain H. pylori antibodies, a coloured line will not appear in this region indicating a negative result. To serve as a procedural control, a coloured line will always appear in the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred. 

The serological approaches (such as the quick immunochemical test described above) detect the reaction of patient's immunologic system against the disease however these approaches prevent the specialist to distinguish an active infection or the passed infection. In addition, the antibody titre does not change in the course of treatment.